I had pretty good results with single Moxy, two should be more than enough.
If you have Kicker or any erg trainer that can be controlled via power meter it is quite easy to do.
Initially I would avoid doing ramps. Yes they show smo2 at different levels etc, but for initial assessment it will just make you tired and could give you inconsistent results if the raps ae to hard. I would leave that at the end if you need to make final tweaks.
Just as an example I was doing 2x5min intervals at kickr set to 70% of FTP. (or simply intensity that will not make you tired on the last interval)
1. Moxy on VL 2x5min, 1min rest for moxy change.
2. Switch to RF and another 2x5min at the same load,
3. Switch Moxy to BF and another 2x5min.
4. Continue with all other muscles you want to find out on the same day)
(with 2 sensors you can measure 2 muscles at the same time, it will speed up the whole process)
I was doing the same protocol on 2-3 different days to have comparison.
I could easily desaturate VL to 0%, my VM was really weak, and my hamstrings were even weaker. I had problems with sprints after long final race accelerations, I was simply getting dropped at the very end, and not because I was weak, but because I was using just one muscle.
Now I use hamstrings for accelerations and surges, also use my VM way more.
I can’t desaturate my VL to 0% anymore, difference between muscles is not that dramatic.
I remember my first “hamstring” engaging intervals was doing them at 45-50% of my FTP but they felt like VO2max sessions.